The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 24, 2016

Filed:

Dec. 11, 2013
Applicant:

Sarepta Therapeutics, Inc., Corvallis, OR (US);

Inventors:

Patrick L. Iversen, Corvallis, OR (US);

David A. Stein, Corvallis, OR (US);

Assignee:

Sarepta Therapeutics, Inc., Corvallis, OR (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/04 (2006.01); C12N 15/113 (2010.01);
U.S. Cl.
CPC ...
C12N 15/1131 (2013.01); C12N 2310/11 (2013.01); C12N 2310/314 (2013.01); C12N 2310/3145 (2013.01); C12N 2310/3233 (2013.01);
Abstract

A method of inhibiting replication of a flavivirus in animal cells, and an oligonucleotide compound for use in the method are disclosed. The oligonucleotide analog (i) has a nuclease-resistant backbone, (ii) is capable of uptake by the cells, (iii) contains between 8-40 nucleotide bases, and (iv) has a sequence of at least 8 bases complementary to a region of the virus' positive strand RNA genome that includes at least a portion of SEQ ID NOS:1-4. Exposure of cells infected with a flavivirus to the analog is effective to form within the cells, a heteroduplex structure composed of the virus ssRNA and the oligonucleotide, characterized by a Tof dissociation of at least 45° C., and having disrupted base pairing between the virus's 5' and 3′ cyclization sequences.


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