The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 10, 2016
Filed:
Feb. 02, 2001
Lance A. Liotta, Bethesda, MD (US);
Emanuel F. Petricoin, Iii, Dunkirk, MD (US);
Katherine L. Paweletz, Thousand Oaks, CA (US);
Alan R. Day, North Potomac, MD (US);
Lance A. Liotta, Bethesda, MD (US);
Emanuel F. Petricoin, III, Dunkirk, MD (US);
Katherine L. Paweletz, Thousand Oaks, CA (US);
Alan R. Day, North Potomac, MD (US);
Instant Medical Diagnostics, LLC, Potomac, MD (US);
Abstract
An assay device for determining the presence of analytes in a cell lysate comprises a porous support member and a plurality of binding reagents arranged and immobilized at multiple reaction sites on the support member. The binding reagents are selected and arranged to assess the status of a selected cellular signal transduction pathway/protein-protein interactive network. In a further aspect, a method for assessing the status of a signal transduction pathway comprises generating a lysate of cells, the lysate retaining one or more pathway molecules present in one or more states and the pathway molecules reflecting signal transduction events taking place in the cells. The method further includes applying the lysate to an immobilized series of binding reagents which can discriminate the pathway molecules and their states. Binding events between the pathway molecules and the binding reagents are identified and the state of the selected signal pathway is determined.