The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 10, 2016

Filed:

Feb. 21, 2011
Applicants:

Yuzuru Takamura, Nomi, JP;

Kotaro Idegami, Nomi, JP;

Mieko Kogi, Nonoichi, JP;

Haruo Takabayashi, Ishikawa, JP;

Inventors:

Yuzuru Takamura, Nomi, JP;

Kotaro Idegami, Nomi, JP;

Mieko Kogi, Nonoichi, JP;

Haruo Takabayashi, Ishikawa, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B03D 3/00 (2006.01); C12N 5/078 (2010.01); C12N 5/00 (2006.01); G01N 33/49 (2006.01); G01N 33/80 (2006.01); C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0641 (2013.01); B03D 3/00 (2013.01); C12N 5/0087 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6883 (2013.01); G01N 33/491 (2013.01); G01N 33/80 (2013.01); C12Q 2600/118 (2013.01); C12Q 2600/158 (2013.01);
Abstract

Method for concentrating and collecting small quantities of fetal nucleated red blood cells contained in the maternal blood. The method for concentrating and collecting nucleated red blood cells from the maternal blood comprises: (i) subjecting the maternal blood to a first density-gradient centrifugation and collecting a cell fraction containing nucleated red blood cells; (ii) treating the cell fraction containing nucleated red blood cells so as to selectively changes the density of the nucleated red blood cells from that of the white blood cells; and (iii) subjecting the treated cell fraction containing the nucleated red blood cells to a second density-gradient centrifugation so as to collect a fraction containing nucleated red blood cells.


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