The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 03, 2016

Filed:

Feb. 20, 2012
Applicants:

Francesca Di Pasquale, Dusseldorf, DE;

Holger Engel, Hilden, DE;

Sascha Strauss, Solingen, DE;

Nicola JO Thelwell, Derbyshire, GB;

Inventors:

Francesca Di Pasquale, Dusseldorf, DE;

Holger Engel, Hilden, DE;

Sascha Strauss, Solingen, DE;

Nicola Jo Thelwell, Derbyshire, GB;

Assignees:

Qiagen GmbH, Hilden, DE;

Qiagen Manchester Limited, Manchester, GB;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6879 (2013.01); C12Q 1/6851 (2013.01); C12Q 1/6888 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01);
Abstract

The present invention relates to a method for quantifying and/or detecting one or more nucleic acids of a genome in a sample, wherein in an amplification reaction, (i) a first nucleic acid is amplified, the locus that is amplified is a multicopy locus (MCL) within the genome, wherein the locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 1 over a stretch of 80 base pairs, and wherein the multicopy locus has copies on at least two different chromosomes, (ii) a second nucleic acid that has been added as an internal control (IC) is also amplified, and (iii) the amount of amplification product from the amplification of the first nucleic acid is determined.


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