The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 03, 2016
Filed:
Apr. 29, 2015
Applicant:
Keygene N.v., Wageningen, NL;
Inventors:
Michael Josephus Theresia Van Eijk, Herpen, NL;
Anker Preben Sørensen, Renkum, NL;
Marco Gerardus Maria Van Schriek, Bennekom, NL;
Assignee:
Keygene N.V., Wageningen, NL;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12P 19/34 (2006.01); C40B 20/00 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6874 (2013.01); C12Q 1/6827 (2013.01); C12Q 1/6855 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6883 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/16 (2013.01);
Abstract
The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.