The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 03, 2016

Filed:

Feb. 05, 2013
Applicant:

Raindance Technologies, Inc., Billerica, MA (US);

Inventors:

Darren Link, Lexington, MA (US);

Laurent Boitard, Gullford, CT (US);

Jeffrey Branciforte, Cromwell, CT (US);

Yves Charles, Waterbury, CT (US);

Gilbert Feke, West Simsbury, CT (US);

John Q. Lu, Gullford, CT (US);

David Marran, Durham, CT (US);

Ahmadali Tabatabai, Branford, CT (US);

Michael Weiner, Guilford, CT (US);

Wolfgang Hinz, Guilford, CT (US);

Jonathan M. Rothberg, Guilford, CT (US);

Assignee:

Raindance Technologies, Inc., Billerica, MA (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); G01N 33/543 (2006.01); B01L 3/00 (2006.01); C12N 15/10 (2006.01); B01F 13/00 (2006.01); B01J 19/00 (2006.01); B82Y 5/00 (2011.01); B82Y 30/00 (2011.01); C07K 1/04 (2006.01); C40B 30/04 (2006.01); C40B 40/04 (2006.01); C40B 50/08 (2006.01); C40B 60/08 (2006.01); C40B 60/12 (2006.01); C40B 70/00 (2006.01); G01N 33/536 (2006.01); G01N 33/68 (2006.01); B01F 3/08 (2006.01); B01L 7/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1075 (2013.01); B01F 13/0071 (2013.01); B01F 13/0076 (2013.01); B01J 19/0046 (2013.01); B01L 3/502784 (2013.01); B82Y 5/00 (2013.01); B82Y 30/00 (2013.01); C07K 1/047 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6874 (2013.01); C12Q 1/6876 (2013.01); C40B 30/04 (2013.01); C40B 40/04 (2013.01); C40B 50/08 (2013.01); C40B 60/08 (2013.01); C40B 60/12 (2013.01); C40B 70/00 (2013.01); G01N 33/536 (2013.01); G01N 33/543 (2013.01); G01N 33/6845 (2013.01); G01N 33/6854 (2013.01); B01F 3/0807 (2013.01); B01J 2219/005 (2013.01); B01J 2219/0072 (2013.01); B01J 2219/00286 (2013.01); B01J 2219/00459 (2013.01); B01J 2219/00466 (2013.01); B01J 2219/00468 (2013.01); B01J 2219/00479 (2013.01); B01J 2219/00576 (2013.01); B01J 2219/00599 (2013.01); B01J 2219/00702 (2013.01); B01J 2219/00743 (2013.01); B01L 3/5027 (2013.01); B01L 3/50273 (2013.01); B01L 3/502746 (2013.01); B01L 3/502761 (2013.01); B01L 3/502792 (2013.01); B01L 3/565 (2013.01); B01L 7/52 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0621 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/0673 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0896 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/0424 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/084 (2013.01); C12Q 1/6869 (2013.01); C12Q 2563/159 (2013.01); C12Q 2565/629 (2013.01); C12Q 2600/16 (2013.01);
Abstract

The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.


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