The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 03, 2016

Filed:

Jun. 26, 2012
Applicants:

Roberto Meidler, Rehovot, IL;

Nina Raver-shapira, Ramot Meir, IL;

Liliana Bar, Rehovot, IL;

Oleg Belyaev, Petah Tikva, IL;

Israel Nur, Moshav Timmorim, IL;

Inventors:

Roberto Meidler, Rehovot, IL;

Nina Raver-Shapira, Ramot Meir, IL;

Liliana Bar, Rehovot, IL;

Oleg Belyaev, Petah Tikva, IL;

Israel Nur, Moshav Timmorim, IL;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/50 (2006.01); C07K 14/81 (2006.01); C12N 9/68 (2006.01); C07K 1/22 (2006.01);
U.S. Cl.
CPC ...
C12N 9/50 (2013.01); C07K 1/22 (2013.01); C07K 14/81 (2013.01); C07K 14/811 (2013.01); C12N 9/6435 (2013.01); C12Y 304/21007 (2013.01);
Abstract

The invention relates to purification of an intact, non-degraded macromolecule from a biological mixture comprising the macromolecule in the presence of its lytic enzyme. The method comprises providing the biological mixture as a heterogeneous mixture comprising the lytic enzyme, at least partially, in soluble form and the macromolecule, at least partially, in non-soluble form; batch-wise contacting the heterogeneous mixture with an immobilized inhibitor of the lytic enzyme; increasing the solubility of the macromolecule in the mixture; and removing the immobilized inhibitor from the mixture.


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