The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 26, 2016

Filed:

Jun. 18, 2009
Applicants:

Blaise D. Porter, Minneapolis, MN (US);

Philippe Levesque, Bloomington, MN (US);

Jacob M. Thibodeau, Minneapolis, MN (US);

Inventors:

Blaise D. Porter, Minneapolis, MN (US);

Philippe Levesque, Bloomington, MN (US);

Jacob M. Thibodeau, Minneapolis, MN (US);

Assignee:

ILLINOIS TOOL WORKS INC., Glenview, IL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/12 (2006.01); C12M 1/00 (2006.01); C12M 1/42 (2006.01);
U.S. Cl.
CPC ...
C12M 23/38 (2013.01); C12M 23/04 (2013.01); C12M 29/10 (2013.01); C12M 35/04 (2013.01);
Abstract

An apparatus and method is described for culturing and conditioning cells on a sample. The apparatus includes a fluid delivery system that transmits gas to a first surface of a sample and transmits liquid to an opposite surface of the sample without transmitting liquid onto the first surface. Further, while culturing cells on the sample, the apparatus enables a constant or variable tension and shear stress applied to the sample. The apparatus may thus, for example, be used to culture cells on an external surface of a tissue construct with air and culture cells underneath the tissue construct with a cell growth media. The fluid delivery system may alternatively transmit liquid cell growth media underneath and on the sides of the tissue construct without flowing onto a designated external surface of the tissue construct. In this manner, cells may be cultured on the tissue construct while stretching and relaxing the tissue and further simulating a dissimilar environment on opposite surfaces of the tissue construct.


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