The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 19, 2016
Filed:
Feb. 06, 2014
Applicants:
Thomas Jostock, Neuenburg am Rhein, DE;
Hans-peter Knopf, Schallstadt, DE;
Inventors:
Thomas Jostock, Neuenburg am Rhein, DE;
Hans-Peter Knopf, Schallstadt, DE;
Assignee:
NOVARTIS AG, Basel, CH;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/06 (2006.01); C12N 1/20 (2006.01); C12N 15/00 (2006.01); C07K 16/18 (2006.01); C07K 16/00 (2006.01); C12P 21/00 (2006.01); C12N 15/67 (2006.01); C12N 15/85 (2006.01);
U.S. Cl.
CPC ...
C12P 21/00 (2013.01); C07K 16/00 (2013.01); C07K 16/18 (2013.01); C12N 15/67 (2013.01); C12N 15/85 (2013.01);
Abstract
The present invention pertains to a method for selecting at least one eukaryotic host cell expressing a product of interest. The method entails providing a plurality of host cells which are dependent upon folate uptake and contain polynucleotides encoding for the product of interest and a DHFR enzyme. The cells are cultured in a selective culture medium containing at least an inhibitor of DHFR and folate. The expressed cells are then selected.