The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 19, 2016

Filed:

Aug. 17, 2015
Applicant:

Ucb Pharma, S.a., Brussels, BE;

Inventors:

Mark Ellis, Slough, GB;

David Paul Humphreys, Slough, GB;

Assignee:

UCB PHARMA, S.A., Brussels, BG;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/21 (2006.01); C12N 1/20 (2006.01); C12R 1/19 (2006.01); C07K 16/24 (2006.01); C12N 9/52 (2006.01); C12N 15/70 (2006.01); C12P 21/02 (2006.01);
U.S. Cl.
CPC ...
C12N 1/20 (2013.01); C07K 16/241 (2013.01); C07K 16/244 (2013.01); C12N 9/52 (2013.01); C12N 15/70 (2013.01); C12P 21/02 (2013.01); C12R 1/19 (2013.01); C07K 2317/14 (2013.01); C07K 2317/55 (2013.01); Y02P 20/52 (2015.11);
Abstract

A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a) a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b) a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c) a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated DegP gene and optionally a polynucleotide sequence encoding a protein of interest.


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