The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 12, 2016
Filed:
Mar. 14, 2013
Life Technologies Corporation, Carlsbad, CA (US);
Bin Li, Palo Alto, CA (US);
Kai Qin Lao, Pleasanton, CA (US);
Jennifer O'Neil, Peabody, MA (US);
Jennifer Kunkel, Amesbury, MA (US);
Kellie Haley, Peabody, MA (US);
Rachel Kasinskas, Amesbury, MA (US);
Zhaochun Ma, Sunnyvale, CA (US);
Pius Brzoska, Woodside, CA (US);
LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US);
Abstract
In some embodiments, the present teachings provide methods for paired end sequencing. In some embodiment, a polynucleotide template to be subjected to paired end sequencing comprises at least one cross linking moiety and at least one scissile moiety. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cleavage step, and (c) a reverse sequencing step. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cross-linking step, (c) a cleavage step, and (d) a reverse sequencing step.