The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 12, 2016
Filed:
Nov. 30, 2010
Applicants:
Takahiro Ochiya, Tokyo, JP;
Masaki Kawamata, Tokyo, JP;
Inventors:
Takahiro Ochiya, Tokyo, JP;
Masaki Kawamata, Tokyo, JP;
Assignees:
National Cancer Center, Tokyo, JP;
DS Pharma Biomedical Co., Ltd., Suita, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2006.01); C12N 15/873 (2010.01); A01K 67/027 (2006.01); C12N 9/22 (2006.01); C12N 15/85 (2006.01); C12N 5/0735 (2010.01);
U.S. Cl.
CPC ...
C12N 15/873 (2013.01); A01K 67/0271 (2013.01); A01K 67/0276 (2013.01); C12N 9/22 (2013.01); C12N 15/8509 (2013.01); A01K 2217/00 (2013.01); A01K 2217/052 (2013.01); A01K 2227/105 (2013.01); A01K 2267/0393 (2013.01); C07K 2319/81 (2013.01); C12N 5/0606 (2013.01); C12N 15/85 (2013.01); C12N 2500/30 (2013.01); C12N 2501/065 (2013.01); C12N 2501/15 (2013.01); C12N 2501/998 (2013.01); C12N 2501/999 (2013.01);
Abstract
The present invention provides a preparation method of a chimeric embryo and a chimeric rat, which is characterized by contacting a rat pluripotent stem cell and a host embryo in the presence of an ES cell differentiation inhibitor. The method includes (a) a step for contacting a fertilized host embryo collected from a female rat and a rat pluripotent stem cell in the presence of an ES cell differentiation suppressant, and (b) a step for culturing the host embryo in contact with the rat pluripotent stem cell to form a chimeric embryo.