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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 9309519 B1

PDFFull Text
Date of Patent:
Apr. 12, 2016

Filed:

Mar. 30, 2010

Method for selecting a high expression recombinant cell line

Applicants:

Myungsam Cho, Incheon, KR;

Min Seok Chang, Incheon, KR;

Jong-mook Kim, Incheon, KR;

Hyunjoo Lee, Incheon, KR;

Yoo Cheol Song, Incheon, KR;

Mansu Kim, Incheon, KR;

Inventors:

MyungSam Cho, Incheon, KR;

Min Seok Chang, Incheon, KR;

Jong-Mook Kim, Incheon, KR;

HyunJoo Lee, Incheon, KR;

Yoo Cheol Song, Incheon, KR;

ManSu Kim, Incheon, KR;

Assignee:

CELLTRION INC., , KR;

Attorney:

Cantor Colburn LLP

Primary Examiner:

Anoop Singh

Assistant Examiner:

David A Montanari

Int. Cl.
CPC ...
C12N 15/65 (2006.01); C12N 15/67 (2006.01); C12N 15/10 (2006.01); C12N 15/85 (2006.01); C12P 21/02 (2006.01); C12N 15/69 (2006.01);
U.S. Cl.
CPC ...
C12N 15/65 (2013.01); C12N 15/1086 (2013.01); C12N 15/69 (2013.01); C12N 15/85 (2013.01); C12P 21/02 (2013.01); C12N 2800/24 (2013.01); C12N 2830/46 (2013.01); C12N 2830/50 (2013.01);
Abstract

The present invention relates to a method of selecting high producer clones by using an expression vector, the expression vector comprising: (i) a gene expression cassette comprising a selectable marker gene to which polyA has been inoperably linked; and (ii) a gene expression cassette which encodes a recombinant protein of interest and to which polyA has been operably linked. According to the invention, high producer clones can be selected from cell populations at least 10 times fewer than in the existing methods of selecting cell lines. Particularly, high producer clones can be selected using a low concentration of MTX compared to a conventional stepwise gene amplification strategy which comprises carrying out multiple amplification steps while increasing the concentration of MTX. Accordingly, the development period of cell lines can be shortened and the labor and cost required for selection of high-productivity cell clones can be reduced, whereby more efficient production of proteins is possible even when general selectable marker genes other than MTX are used.

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