The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 22, 2016

Filed:

Nov. 10, 2009
Applicants:

James Ross, Decatur, GA (US);

Edgar A. Brown, Decatur, GA (US);

Swaminathan Rajaraman, Atlanta, GA (US);

Mark G. Allen, Atlanta, GA (US);

Bruce Wheeler, Gainesville, FL (US);

Inventors:

James Ross, Decatur, GA (US);

Edgar A. Brown, Decatur, GA (US);

Swaminathan Rajaraman, Atlanta, GA (US);

Mark G. Allen, Atlanta, GA (US);

Bruce Wheeler, Gainesville, FL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/487 (2006.01); C12N 13/00 (2006.01); C12M 1/42 (2006.01); G01N 33/483 (2006.01);
U.S. Cl.
CPC ...
C12N 13/00 (2013.01); C12M 1/42 (2013.01); G01N 33/4836 (2013.01); G01N 33/48728 (2013.01); B32B 2535/00 (2013.01); G01N 33/48735 (2013.01); Y10T 156/10 (2015.01);
Abstract

Provided herein are apparatus and methods relating to the development of instrumentation for high throughput network electrophysiology and cellular analysis. More specifically, provided herein are multiwell microelectrode arrays (MEAs) and methods for the development of such an apparatus in an inexpensive fashion with a flexible, ANSI/SBS-compliant (American National Standards Institute/Society for Biomolecular Screening) format. Microelectrode arrays are a grid of tightly spaced microelectrodes useful for stimulating and sensing electrically active cells, networks and tissue. The techniques described herein relate to the use of microfabrication in combination with certain large-area processes that have been employed to achieve multiwell MEAs in ANSI/SBS-compliant culture well formats, which are also transparent for inverted/backside microscopy compatibility. These multiwell MEAs can be used to investigate two and three-dimensional networks of electrically active cells and tissue such as cardiac, neural, and muscular in a high throughput fashion. Also being ANSI/SBS-compliant, they are compatible with machinery and robotics developed for the pharmaceutical industry for drug screening applications.


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