The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 22, 2016

Filed:

Feb. 05, 2009
Applicants:

Masahiro Sokabe, Nagoya, JP;

Kishio Furuya, Nagoya, JP;

Go Tazaki, Tsukuba, JP;

Hitoshi Tsuruta, Tsukuba, JP;

Motohiro Fukuda, Tsukuba, JP;

Inventors:

Masahiro Sokabe, Nagoya, JP;

Kishio Furuya, Nagoya, JP;

Go Tazaki, Tsukuba, JP;

Hitoshi Tsuruta, Tsukuba, JP;

Motohiro Fukuda, Tsukuba, JP;

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); C12N 5/00 (2006.01); C12M 1/32 (2006.01); C12M 3/06 (2006.01); C12M 1/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0631 (2013.01); C12M 23/12 (2013.01); C12M 23/16 (2013.01); C12M 23/22 (2013.01); C12N 5/0062 (2013.01); C12N 2535/10 (2013.01);
Abstract

To provide a cell culture method capable of sustaining in-vivo functions for a long time by suppressing dedifferentiation. A cell culture method in accordance with the present invention is to culture cells in a multi-layered state in a minute partitioned space and to obtain a tissue structure having a function resembling an in-vivo function. When the cells are mammary gland epithelial cells, a hollow acinus-like structure can be formed. The minute partitioned space is particularly preferably a micro container in a cell culture container having a plurality of such micro containers on the surface.


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