The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 22, 2016

Filed:

Sep. 14, 2011
Applicants:

Hidekazu Sekine, Shinjuku-ku Tokyo, JP;

Tatsuya Shimizu, Shinjuku-ku Tokyo, JP;

Teruo Okano, Shinjuku-ku Tokyo, JP;

Inventors:

Hidekazu Sekine, Shinjuku-ku Tokyo, JP;

Tatsuya Shimizu, Shinjuku-ku Tokyo, JP;

Teruo Okano, Shinjuku-ku Tokyo, JP;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A61K 35/44 (2015.01); A61K 35/34 (2015.01); A61K 35/36 (2015.01); C12N 5/077 (2010.01); A61L 27/36 (2006.01); A61L 27/38 (2006.01);
U.S. Cl.
CPC ...
A61K 35/44 (2013.01); A61K 35/34 (2013.01); A61K 35/36 (2013.01); A61L 27/3604 (2013.01); A61L 27/3839 (2013.01); A61L 27/3895 (2013.01); C12N 5/0657 (2013.01); C12N 2502/28 (2013.01); C12N 2513/00 (2013.01);
Abstract

A method for producing multilayered cell sheets, including producing a vascular bed which includes an artery-vein loop and in which a capillary vascular network is constructed; layering cell sheets on the vascular bed; and perfusing a culture medium in vitro to construct a vascular network in the cell sheets. The production method enables vascular networks to be constructed in cell sheets and enables thick multilayered cell sheets to foe easily produced by layering the cell sheets. Such thick multilayered cell sheets are useful as in vivo tissue-like products for regenerative medicine for various tissues and for evaluation of drugs and the like.


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