The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 15, 2016

Filed:

Oct. 06, 2011
Applicants:

Alice Y. Ting, Allston, MA (US);

David Baker, Seattle, WA (US);

Florian Richter, Seattle, WA (US);

Lucas Nivon, Seattle, WA (US);

Daniel Shao-chen Liu, Cambridge, MA (US);

Inventors:

Alice Y. Ting, Allston, MA (US);

David Baker, Seattle, WA (US);

Florian Richter, Seattle, WA (US);

Lucas Nivon, Seattle, WA (US);

Daniel Shao-Chen Liu, Cambridge, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/12 (2006.01); C12N 15/00 (2006.01); C07H 21/04 (2006.01); C12N 15/10 (2006.01); C12N 9/00 (2006.01); G01N 33/532 (2006.01); G01N 33/533 (2006.01);
U.S. Cl.
CPC ...
C12N 9/1241 (2013.01); C12N 9/93 (2013.01); C12N 15/102 (2013.01); C12Y 207/07063 (2013.01); G01N 33/532 (2013.01); G01N 33/533 (2013.01); G01N 2333/9015 (2013.01);
Abstract

The present disclosure provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ lipoic acid ligase or mutants thereof, and lipoic acid analogs (e.g., lipoic acid analogs comprising a resorufin moiety) recognized by lipoic acid ligase and lipoic acid ligase mutants. Also provided herein is a method of imaging protein-protein interaction via a reaction mediated by lipoic acid ligase.


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