The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 08, 2016
Filed:
Feb. 27, 2013
National Institute of Advanced Industrial Science and Technology, Tokyo, JP;
Wako Pure Chemical Industries, Ltd., Osaka, JP;
Hiroaki Tateno, Ibaraki, JP;
Jun Hirabayashi, Ibaraki, JP;
Makoto Asashima, Ibaraki, JP;
Yuzuru Ito, Ibaraki, JP;
Yasuko Onuma, Ibaraki, JP;
Masaki Warashina, Hyogo, JP;
Masakazu Fukuda, Hyogo, JP;
NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY, Tokyo, JP;
WAKO PURE CHEMICAL INDUSTRIES, LTD., Osaka, JP;
Abstract
Provided are a method for accurately evaluating the differentiation status of stem cells by selectively staining only stem cells in an undifferentiated state, and a method for positively isolating only stem cells in an undifferentiated state. Specifically provided is a method for determining differentiation of a cell comprising a step of contacting a test cell with a probe comprising protein (A) or (B) below and a step of detecting the presence of binding of the probe to the test cell. The method for determining differentiation of a cell is capable of detecting the presence or absence of an undifferentiated stem cell in test cells by using a probe that specifically reacts with undifferentiated stem cells and detecting the presence of bonding to the test cell. (A) A protein comprising an amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of 'Fucα1-2Galβ1-3GlcNAc' and/or 'Fucα1-2Galβ1-3GalNAc;' and (B) a protein comprising an amino acid sequence showing 80% or more similarity to the amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of “Fucα1-2Galβ1-3GlcNAc” and/or “Fucα1-2Galβ1-3GalNAc.”