The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 08, 2016

Filed:

Aug. 24, 2012
Applicants:

Jacqueline Montalibet, Manotick, CA;

Loreta Gudynaite-savitch, Kanata, CA;

Christopher Hill, Nepean, CA;

Christopher D. Hindle, Gloucester, CA;

James A. Lavigne, Nepean, CA;

Nabil Masri, Gatineau, CA;

Fuad Tahna, Ottawa, CA;

John J. Tomashek, Ottawa, CA;

Inventors:

Jacqueline Montalibet, Manotick, CA;

Loreta Gudynaite-Savitch, Kanata, CA;

Christopher Hill, Nepean, CA;

Christopher D. Hindle, Gloucester, CA;

James A. Lavigne, Nepean, CA;

Nabil Masri, Gatineau, CA;

Fuad Tahna, Ottawa, CA;

John J. Tomashek, Ottawa, CA;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/42 (2006.01); C12N 15/80 (2006.01); C12P 19/14 (2006.01);
U.S. Cl.
CPC ...
C12Y 302/01091 (2013.01); C12N 9/2437 (2013.01); C12N 15/80 (2013.01); C12P 19/14 (2013.01);
Abstract

Provided are isolated cellobiohydrolases comprising a modified Glycoside Hydrolase (GH) Family 7 catalytic domain, a GH Family 7 catalytic domain and a modified Family 1 carbohydrate binding module (CBM), or both a modified Family 7 catalytic domain and a modified Family 1 CBM. Such isolated cellobiohydrolases exhibit from 45% to about 99.9% amino acid sequence identity to amino acids 1-436 of SEQ ID NO: 1 or to amino acids 1-438 of SEQ ID NO: 2 and improved activity on process substrates. Also provided are genetic constructs and genetically modified microbes for expressing the isolated cellobiohydrolases, a process for producing the isolated cellobiohydrolases, cellulase enzyme mixtures comprising the isolated cellobiohydrolase and a process for hydrolyzing a cellulosic substrate with such cellulase enzyme mixtures.


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