The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 08, 2016
Filed:
Jan. 23, 2012
Kazutoshi Mihara, Hyogo, JP;
Atsuko Kawasaki, Hyogo, JP;
Kouta Ootsuki, Hyogo, JP;
Yuukichi Hatano, Hyogo, JP;
Shoichiro Kamei, Hyogo, JP;
Atsushi Sugimura, Hyogo, JP;
Kazutoshi Mihara, Hyogo, JP;
Atsuko Kawasaki, Hyogo, JP;
Kouta Ootsuki, Hyogo, JP;
Yuukichi Hatano, Hyogo, JP;
Shoichiro Kamei, Hyogo, JP;
Atsushi Sugimura, Hyogo, JP;
JCR Pharmaceuticals Co., Ltd., Ashiya-shi, JP;
Abstract
Disclosed is a method for production of recombinant human iduronate 2-sulfatase (rhI2S) (the 26th to 550th amino acids of SEQ ID NO: 10) in a large scale, with a high purity, and mannose 6-phosphate residues. The method comprises the steps of (a) culturing rhI2S (the 26th to 550th amino acids of SEQ ID NO: 10)-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) to dye affinity column chromatography, (e) to anion-exchange column chromatography, and (f) to a column chromatography employing as solid phase a material having affinity for phosphate group, and (g) to gel filtration column chromatography, in the order.