The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 01, 2016

Filed:

May. 23, 2007
Applicants:

Paul Van Bilsen, Maastricht, NL;

William F. Kaemmerer, Edina, MN (US);

Eric Burright, Eagan, MN (US);

Inventors:

Paul van Bilsen, Maastricht, NL;

William F. Kaemmerer, Edina, MN (US);

Eric Burright, Eagan, MN (US);

Assignee:

Medtronic, Inc., Minneapolis, MN (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C07H 21/04 (2006.01); C12N 15/113 (2010.01);
U.S. Cl.
CPC ...
C12Q 1/6883 (2013.01); C12N 15/113 (2013.01); C12Q 1/6809 (2013.01); C12Q 1/6827 (2013.01); C12N 2310/14 (2013.01); C12N 2320/34 (2013.01); C12Q 2600/156 (2013.01);
Abstract

Methods and kits are provided for determining which single nucleotide polymorphism ('SNP') variant of an allele of a heterozygous patient is on the same allele as a disease-causing mutation that is at a remote region of the gene's mRNA comprising a) an allele specific reverse transcription reaction using an allele specific primer which recognizes one SNP variant, wherein further the 3' end of the primer is positioned at the SNP nucleotide position, and b) analysis of the resulting cDNA product from the reverse transcription reaction at the region of the mutation to determine the presence or absence of the mutation on this allele specific cDNA product, wherein the allele specific primer is shorter than about 20 nucleotides.


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