The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 01, 2016
Filed:
Jun. 21, 2011
Ritu Bhalla, New Delhi, IN;
Qi Chen, Wallingford, PA (US);
Qiong Cheng, Wilmington, DE (US);
Neeraj Pandey, Haryana, IN;
Pierre E. Rouviere, Wilmington, DE (US);
Kristin Ruebling-jass, Wilmington, DE (US);
Annapurna Sachan, Kanpur, IN;
Ritu Bhalla, New Delhi, IN;
Qi Chen, Wallingford, PA (US);
Qiong Cheng, Wilmington, DE (US);
Neeraj Pandey, Haryana, IN;
Pierre E. Rouviere, Wilmington, DE (US);
Kristin Ruebling-Jass, Wilmington, DE (US);
Annapurna Sachan, Kanpur, IN;
E. I. du Pont de Nemours and Company, Wilmington, DE (US);
Abstract
Methods are provided to obtain recombinant microbial cells having at least one genetic modification that increase the buoyant density of a recombinant microbial cell or the buoyant density of inclusion bodies produced within a recombinant microbial cell. Exemplified are genetic modifications that increase the buoyant density of a recombinant microbial cell expressing heterologous peptides and polypeptides. Increasing expression of the genes ysaB, glyQ, glyS or a combination thereof within the recombinant microbial cell produces cells or inclusion bodies having higher buoyant density. A similar effect was achieved by decreasing or disrupting expression of the endogenous gltA gene. Increases in buoyant density render peptide production more efficient with respect to time and costs.