The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 01, 2016

Filed:

Jan. 22, 2010
Applicants:

Daniel Metzger, Strasbourg, FR;

Pierre Chambon, Blaesheim, FR;

Huimin Zhao, Champaign, IL (US);

John Katzenellenbogen, Urbana, IL (US);

Inventors:

Daniel Metzger, Strasbourg, FR;

Pierre Chambon, Blaesheim, FR;

Huimin Zhao, Champaign, IL (US);

John Katzenellenbogen, Urbana, IL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2006.01); C07K 14/72 (2006.01); C12N 9/90 (2006.01);
U.S. Cl.
CPC ...
C07K 14/721 (2013.01); C12N 9/90 (2013.01); C07K 2319/715 (2013.01);
Abstract

The present invention relates to a method for tightly temporally controlling the biological activity of a protein of interest in a vertebrate, upon induction of the activity of a fusion protein comprising said protein of interest and an ERM polypeptide containing a mutated ligand binding domain of the human oestrogen receptor α, with a synthetic ligand that does not interfere with oestrogen signalling. In particular, the present invention concerns a method for generating tightly temporally-controlled targeted somatic mutations in a vertebrate, preferably a mouse, by inducing the activity of a fusion protein comprising a site-specific recombinase protein and an ERM polypeptide, with a synthetic ligand devoid of oestrogenic and anti-oestrogenic activities.


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