The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 16, 2016

Filed:

Nov. 04, 2010
Applicants:

Dong Ki Lee, Seoul, KR;

Dua Pooja, Gyeonggi-do, KR;

Inventors:

Dong Ki Lee, Seoul, KR;

Dua Pooja, Gyeonggi-do, KR;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/04 (2006.01); C07H 21/02 (2006.01); C12N 15/11 (2006.01); C12N 15/113 (2010.01);
U.S. Cl.
CPC ...
C07H 21/02 (2013.01); C12N 15/111 (2013.01); C12N 15/113 (2013.01); C12N 2310/14 (2013.01); C12N 2310/533 (2013.01); C12N 2320/53 (2013.01);
Abstract

The present invention relates to a novel siRNA structure and the use thereof, and more particularly to a double-stranded siRNA molecule comprising an antisense strand and a sense strand, wherein the siRNA molecule has at least one single nucleotide bulge formed by introducing a single nucleotide into the antisense strand, particularly at position 2 from the 5' end, and to a method of using the same to silence a target gene. The siRNA molecule of the invention shows high target gene silencing efficiency while minimizing off-target effects caused by the antisense strand, and thus has improved target selectivity. Accordingly, the siRNA molecule of the invention can be substituted for conventional siRNA molecules and can be widely be used in siRNA-based gene silencing techniques, including gene therapy.


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