The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 02, 2016

Filed:

Jun. 06, 2014
Applicant:

Pierce Biotechnology, Inc., Rockford, IL (US);

Inventors:

Greg Hermanson, Loves Park, IL (US);

John Charles Rogers, Rockton, IL (US);

Joel Louette, Ulm, DE;

Ryan Daniel Bomgarden, Winnebago, IL (US);

Bhavinkumar Patel, Rockford, IL (US);

Assignee:

Pierce Biotechnology, Inc., Rockford, IL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 31/00 (2006.01); G01N 33/53 (2006.01); H01J 49/00 (2006.01); G01N 33/60 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
H01J 49/0031 (2013.01); G01N 33/60 (2013.01); G01N 33/6848 (2013.01);
Abstract

A method for absolute protein or peptide quantitation by mass spectroscopy. A sample containing a protein or peptide of interest is prepared for mass spectroscopy analysis. The sample is subjected to mass spectroscopy analysis at low resolution whereby a single additive mass spectroscopy peak is obtained, then is subjected to high resolution mass spectroscopy analysis whereby a plurality of mass spectroscopy peaks are obtained. The intensity of each of the plurality of mass spectroscopy peaks is quantitated either by comparison to an internal standard set, or by using a standard curve generated for each isotopologue set. Quantitation using a standard curve enhances quantitation across a dynamic range of analyte.


Find Patent Forward Citations

Loading…