The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 9249457 B1

PDF
Full Text
Expired
Date of Patent:
Feb. 02, 2016

Filed:

Jun. 18, 2009
Applicants:

Piero Carninci, Yokohama, JP;

Charles Plessy, Yokohama, JP;

Roberto Simone, Yokohama, JP;

Inventors:

Piero Carninci, Yokohama, JP;

Charles Plessy, Yokohama, JP;

Roberto Simone, Yokohama, JP;

Assignee:

Riken, Wako-shi, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/02 (2006.01); C12Q 1/68 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6848 (2013.01); C07H 21/02 (2013.01); C12P 19/34 (2013.01); C12Q 1/68 (2013.01);
Abstract

The purpose of the subject invention is to provide a method of manufacturing a mixture of amplified double-stranded nucleic acids comprising unknown sequence including the complete 5' end sequence. A method of manufacturing a mixture of amplified double-stranded nucleic acids comprising: (a) preparing a single-stranded nucleic acid comprising a single-stranded adapter 1, a single-stranded nucleic acid fragment and a single-stranded adapter 2, and (b) conducting PCR with said single-stranded nucleic acid prepared in step (a), a primer 1, and a primer 2 to amplify double-stranded nucleic acids.


Find Patent Forward Citations

Carninci, Piero. (2016). Method of manufacturing a mixture of amplified double-stranded nucleic acids comprising unknown sequence (U.S. Patent No. 9249457). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/9249457

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

Loading…