The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 26, 2016
Filed:
May. 14, 2012
Michael G. Pollack, Crumpler, NC (US);
Vamsee K. Pamula, Cary, NC (US);
Richard B. Fair, Durham, NC (US);
Peter B. Griffin, Woodside, CA (US);
Michael G. Pollack, Crumpler, NC (US);
Vamsee K. Pamula, Cary, NC (US);
Richard B. Fair, Durham, NC (US);
Peter B. Griffin, Woodside, CA (US);
ADVANCED LIQUID LOGIC, INC, San Diego, CA (US);
DUKE UNIVERSITY, Durham, NC (US);
THE BOARD OF TRUSTEES OF THE LELAND STANDFORD JUNIOR UNIVERSITY, Palo Alto, CA (US);
Abstract
The present invention relates to droplet-based pyrosequencing including a method of identifying a base at a target position in a sample nucleic acid. The method includes: (a) providing a droplet microactuator including a first droplet including a sample nucleic acid immobilized on a bead; and (b) on the droplet microactuator: (i) contacting the first droplet with one or more reagent droplets to yield a second droplet, wherein the one or more reagent droplets include reagents for extending a double stranded portion of the sample nucleic acid by incorporating a nucleotide at the target position; (ii) splitting the second droplet to yield a third droplet including the bead and a fourth droplet lacking the bead; and (iii) assaying the third droplet to determine whether the nucleotide was incorporated at the target position.