The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 19, 2016
Filed:
Dec. 02, 2011
Applicant:
Lloyd Ruddock, Oulu, FI;
Inventor:
Lloyd Ruddock, Oulu, FI;
Assignee:
Univeristy of Oulu, Oulu, FI;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/06 (2006.01); C12N 15/70 (2006.01); C12N 9/02 (2006.01); C12N 9/90 (2006.01); C12P 21/02 (2006.01);
U.S. Cl.
CPC ...
C12N 15/70 (2013.01); C12N 9/0051 (2013.01); C12N 9/90 (2013.01); C12P 21/02 (2013.01); C12Y 108/03002 (2013.01); C12Y 503/04001 (2013.01);
Abstract
The present invention relates to a method for producing a protein of interest containing one or more disulfide bonds in its native state. The method comprises that a prokaryotic host cell is genetically engineered to express the protein of interest and a sulfhydryl oxidase in the cytoplasm of the host cell. The protein of interest is formed in a soluble form and contains disulfide bonds due to the presence of the sulfhydryl oxidase in the cytoplasm of said host cell. The present invention relates also to a prokaryotic host cell and a vector system for producing a protein of interest containing natively folded disulfide bonds.