The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 12, 2016
Filed:
Oct. 14, 2009
Wayne M. Barnes, University City, MO (US);
Wayne M. Barnes, University City, MO (US);
Other;
Abstract
Provided herein is a method for homologous end cloning using a 5'-exonuclease. One aspect provides a method of generating a recombinant DNA molecule comprising 5′ exonuclease digestion of DNA molecules, exonuclease inactivation, annealing of 3′ overhangs to form a annealed complex. Also provided is a method a method of generating a recombinant DNA molecule comprising 5′ exonuclease digestion of DNA molecules, exonuclease inactivation, annealing of 3′ overhangs to form a annealed complex, and extension of 3′ ends to fill gaps corresponding to exonuclease removal to form a substantially non-gapped annealed complex. Also provided is a one reaction method in which no further additions need be made to the reaction mixture and the reaction mixture is only manipulated thereafter as to incubation time and incubation temperature. Complexes formed according to methods described herein can be transformed into a host cell without further in vitro processing.