The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 05, 2016

Filed:

May. 24, 2013
Applicant:

Rutgers, the State University of New Jersey, New Brunswick, NJ (US);

Inventors:

Gaetano T. Montelione, Highland Park, NJ (US);

Masayori Inouye, New Brunswick, NJ (US);

Yuefeng Tang, Highland Park, NJ (US);

Monica Roth, New York, NY (US);

William Schneider, Hamilton, NJ (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/06 (2006.01); C12N 1/20 (2006.01); C12N 15/00 (2006.01); C12P 21/00 (2006.01); C12N 9/22 (2006.01); C12P 21/02 (2006.01); G01N 33/534 (2006.01); G01N 33/60 (2006.01); C12N 15/63 (2006.01); G01N 24/08 (2006.01);
U.S. Cl.
CPC ...
C12P 21/00 (2013.01); C12N 9/22 (2013.01); C12N 15/635 (2013.01); C12P 21/02 (2013.01); G01N 24/087 (2013.01); G01N 33/534 (2013.01); G01N 33/60 (2013.01); G01N 2333/922 (2013.01);
Abstract

Disclosed herein is a set ofsingle-protein production (SPP) technologies with protein NMR (SPP-NMR) for (i) using isotope-enriched membrane proteins produced with the SPP system in screening detergent conditions suitable for purification and/or three-dimensional structure analysis without the requirement for protein purification, (ii) producingH,C,N enriched proteins suitable for high throughput and membrane protein NMR studies, and (iii) labeling withC—N specific peptide bonds in proteins (referred to herein as SPP-PBL).


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