The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 05, 2016
Filed:
Jun. 01, 2009
Charles Abbas, Champaign, IL (US);
Kostyantyn Dmytruk, Lviv, GB;
Olena Dmytruk, Lviv, GB;
Andriy Sibirny, Lviv, GB;
Andriy Y. Voronovsky, Lviv, GB;
Charles Abbas, Champaign, IL (US);
Kostyantyn Dmytruk, Lviv, GB;
Olena Dmytruk, Lviv, GB;
Andriy Sibirny, Lviv, GB;
Andriy Y. Voronovsky, Lviv, GB;
Archer Daniels Midland Co., Decatur, IL (US);
Abstract
Recombinant genetic constructs and strains ofhaving significantly increased ethanol productivity with a simultaneous decreased production of xylitol during high-temperature xylose fermentation are disclosed. The constructs include aXYL1 gene encoding xylose reductase mutated to decrease affinity of the enzyme toward NADPH. The modified version of XYL1 gene under control of a strong constitutive HpGAP promoter was overexpressed in a Δxyl1 background. A recombinantstrain overexpressing the mutated enzyme together with native xylitol dehydrogenase and xylulokinase in the Δxyl1 background was also constructed. Xylose consumption, ethanol and xylitol production by the constructed strain were evaluated during high-temperature xylose fermentation (48° C.). A significant increase in ethanol productivity (up to 7.4 times) was shown in the recombinant strain as compared with the wild type strain. Moreover, the xylitol production by the recombinant strain was reduced considerably: 0.9 mgχ (Lχh)versus 4.2 mgχ (Lxh)by the wild type strain.