The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 29, 2015

Filed:

Aug. 12, 2003
Applicants:

Christopher L. Baszczynski, Earlham, IA (US);

Benjamin A. Bowen, Berkeley, CA (US);

David J. Peterson, Ames, IA (US);

Laura Tagliani, Zionsville, IN (US);

Inventors:

Christopher L. Baszczynski, Earlham, IA (US);

Benjamin A. Bowen, Berkeley, CA (US);

David J. Peterson, Ames, IA (US);

Laura Tagliani, Zionsville, IN (US);

Assignee:

Other;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/82 (2006.01); C12N 9/00 (2006.01); C12N 15/10 (2006.01); C12N 15/113 (2010.01); C12N 15/90 (2006.01);
U.S. Cl.
CPC ...
C12N 15/8216 (2013.01); C12N 9/00 (2013.01); C12N 15/102 (2013.01); C12N 15/1137 (2013.01); C12N 15/8203 (2013.01); C12N 15/8205 (2013.01); C12N 15/8213 (2013.01); C12N 15/8274 (2013.01); C12N 15/90 (2013.01); C07K 2319/00 (2013.01); C12N 2310/321 (2013.01); C12N 2310/53 (2013.01);
Abstract

Compositions for the targeted integration of nucleotide sequences into a plant are provided. Plants and plant cells having multiple transfer cassettes stacked at precise locations in the genome of a plant or plant cell are provided. In specific embodiments, the plant or plant cell comprises at least two transfer cassettes. The first transfer cassette comprises a first nucleotide sequence of interest flanked by a first and a second recombination site, wherein the first and the second recombination sites are non-identical. The second transfer cassette comprises a second nucleotide sequence of interest flanked by the second recombination site and a third recombination site, wherein the third recombination site is non-identical to the first and said second recombination site. The second recombination site is shared between the first and the second transfer cassette, such that the genome of the plant cell comprises in the following order the first recombination site, the first nucleotide sequence of interest, the second recombination site, the second nucleotide sequence of interest, and the third recombination site.


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