The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 29, 2015

Filed:

Oct. 04, 2011
Applicants:

Meir Israelowitz, Toronto, CA;

Birgit Weyand, Hannover, DE;

Peter Vogt, Hannover, DE;

Kerstin Reimers, Hannover, DE;

Herbert Von Schroeder, Toronto, CA;

Christoph Gille, Toronto, CA;

Elmar Schmaelzlin, Postdam, DE;

Inventors:

Meir Israelowitz, Toronto, CA;

Birgit Weyand, Hannover, DE;

Peter Vogt, Hannover, DE;

Kerstin Reimers, Hannover, DE;

Herbert von Schroeder, Toronto, CA;

Christoph Gille, Toronto, CA;

Elmar Schmaelzlin, Postdam, DE;

Assignees:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 3/00 (2006.01); G01N 33/84 (2006.01); C12N 5/0775 (2010.01); C12N 5/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0675 (2013.01); C12N 5/0062 (2013.01); C12N 5/0662 (2013.01); C12N 5/0663 (2013.01); C12N 5/0664 (2013.01); C12N 5/0665 (2013.01); C12N 5/0666 (2013.01); C12N 5/0667 (2013.01); C12N 5/0668 (2013.01); C12Q 3/00 (2013.01); G01N 33/84 (2013.01); C12N 2500/02 (2013.01);
Abstract

The invention provides a process for producing a three-dimensional tissue by cultivating eucaryotic cells by introducing the cells into a matrix and cultivating the cells within the matrix in a cell culture medium within a cell culture vessel under controlled dissolved oxygen conditions of the cell culture medium. The matrix contains a support containing an optical oxygen sensor, which is an oxygen-sensitive dye, which upon irradiation with an excitation wavelength changes its emission characteristics in dependence on the dissolved oxygen concentration in the surrounding medium, including a dye phosphorescing upon irradiation of an excitation wavelength, which phosphorescence is quenched by dissolved oxygen.


Find Patent Forward Citations

Loading…