The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 29, 2015

Filed:

Jun. 23, 2008
Applicants:

James W. Schneider, Pittsburgh, PA (US);

Shane T. Grosser, Princeton, NJ (US);

Jeffrey M. Savard, McGaheysville, VA (US);

Inventors:

James W. Schneider, Pittsburgh, PA (US);

Shane T. Grosser, Princeton, NJ (US);

Jeffrey M. Savard, McGaheysville, VA (US);

Assignee:

CARNEGIE MELLON UNIVERSITY, Pittsburgh, PA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 27/26 (2006.01); C07H 21/00 (2006.01); C07K 1/00 (2006.01); C07H 19/04 (2006.01); G01N 30/58 (2006.01);
U.S. Cl.
CPC ...
C07H 19/04 (2013.01); G01N 2030/582 (2013.01);
Abstract

The invention relates transiently attaching drag-tags to molecules during electrophoresis. The invention includes running buffers having drag-tags that transiently attach to lipophilic moieties attached to the molecules. The lipophilic moieties can be covalently or ionically bonded to the molecules. One particular aspect of the invention is a nucleoside analog or a nucleic acid analog comprising a lipophilic moiety. The invention is also directed to methods of separating molecules that comprise a lipophilic moiety. The methods generally comprise transiently attaching a drag-tag to the lipophilic moiety during a separation modality. These methods can be used to separate the molecules by size or weight, to measure a hydrodynamic radius of a drag-tag, or to separate a plurality of drag-tag by their hydrodynamic radius.


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