The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 22, 2015
Filed:
Apr. 25, 2014
Applicant:
Handylab, Inc., Ann Arbor, MI (US);
Inventors:
Sundaresh N. Brahmasandra, Ann Arbor, MI (US);
Elizabeth Craig, Ypsilanti, MI (US);
Assignee:
HANDYLAB, INC., Ann Arbor, MI (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/00 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2006.01); C07H 1/08 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1013 (2013.01); C07H 1/08 (2013.01); C12Q 1/6813 (2013.01);
Abstract
Disclosed are methods for isolating polynucleotides (e.g., RNA and DNA) from a cell-containing sample. The disclosed methods employ polyamidoamine (PAMAM) dendrimer molecules bound to the surface of binding particles to capture and isolate the polynucleotides from the samples. The polynucleotides are released from the binding particles by contacting the binding particles with a release solution having a basic pH.