The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 22, 2015

Filed:

Sep. 08, 2011
Applicants:

Junji Fukuda, Yokohama, JP;

Hiroaki Suzuki, Ibaraki, JP;

Naoto Mochizuki, Ibaraki, JP;

Takahiro Kakegawa, Ibaraki, JP;

Inventors:

Junji Fukuda, Yokohama, JP;

Hiroaki Suzuki, Ibaraki, JP;

Naoto Mochizuki, Ibaraki, JP;

Takahiro Kakegawa, Ibaraki, JP;

Assignee:

UNIVERSITY OF TSUKUBA, Tsukuba, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 11/02 (2006.01); C12M 1/32 (2006.01); C12M 1/26 (2006.01); C12N 5/00 (2006.01); C12M 1/12 (2006.01);
U.S. Cl.
CPC ...
C12N 11/02 (2013.01); C12M 23/12 (2013.01); C12M 25/08 (2013.01); C12M 33/00 (2013.01); C12N 5/0068 (2013.01); C12N 2509/00 (2013.01); C12N 2529/00 (2013.01); C12N 2533/50 (2013.01);
Abstract

There is provided a culture method and a culture device for efficiently producing cells and/or cell tissue suitable for medication applications. The culture method may include a first step of adhering the cells onto the electrode layer and culturing the cells thereon. The electrode layer may be coated with an oligopeptide including a terminal amino acid, a cell adhesive sequence, and an alternating sequence to be bound to the one end side of the cell-adhesive sequence. The alternating sequence may include a plurality of acidic amino acids and a plurality of basic amino acids that are alternately bound to each other one by one. The culture method may include a second step of applying, to the electrode layer having the cells adhered thereonto, an electrical potential inducing reductive desorption of the oligopeptide, to thereby detach the cells from the electrode layer.


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