The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 9217139 B1

PDF
Full Text
Expired
Date of Patent:
Dec. 22, 2015

Filed:

Sep. 15, 2011
Applicants:

Kozue Mori, Yokosuka, JP;

Yukari Ohta, Yokosuka, JP;

Yuji Hatada, Yokosuka, JP;

Nobuyuki Nakamura, Yokosuka, JP;

Masayuki Miyazaki, Yokosuka, JP;

Inventors:

Kozue Mori, Yokosuka, JP;

Yukari Ohta, Yokosuka, JP;

Yuji Hatada, Yokosuka, JP;

Nobuyuki Nakamura, Yokosuka, JP;

Masayuki Miyazaki, Yokosuka, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/22 (2006.01); C12R 1/465 (2006.01); C12Q 1/44 (2006.01); C12N 1/08 (2006.01);
U.S. Cl.
CPC ...
C12N 9/22 (2013.01); C12N 1/08 (2013.01); C12Q 1/44 (2013.01); C12R 1/465 (2013.01);
Abstract

The purpose of the present invention is to provide a nuclease that secretes natural nonpathogenic microorganisms extracellularly, has higher specific activity than conventional nucleases, and is useful in nucleolytic degradation on an industrial scale. This purpose is achieved with an extracellularly secreted nuclease derived frombacteria, the nuclease having specific activity equal to or greater than the specific activity of Benzonase® when supplied to double-stranded DNA for 30 minutes at 37° C. in 20 mM Tris/HCl (pH 8.5) containing 1 mM MgCland 1 mM CaClafter purification, using double-stranded DNA, single-stranded DNA, and RNA as substrates.


Find Patent Forward Citations

Mori, Kozue. (2015). Extracellularly secreted nuclease (U.S. Patent No. 9217139). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/9217139

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