Microfluidic devices and methods of manufacture and use

Abstract

Series of bio-detection elements, shown as short hollow transparent reaction vessels (), batch-surface-coated with capture-agent, are placed in channel of larger width (a; c), and fixed by flexible or elastomeric over-lying sheet (a; c). Portions of sheet form pneumatically-activated pistons () and valves (-) to produce and control channel flow. Overlying sheet is of reflectively-coated Mylar (b) or PDMS, bonded with channel-defining structure. PDMS sheet is surface-activated for molecular-bond with PDMS channel-defining structure, molecular-bond with PDMS-valve-seat-forming portions () defeated by repeated make-and-break contact during cure. Pick-and-place of detection elements in channel employs electrostatic attraction to seize elements, enabling tool removal. Rigidly-backed parts are brought together to channel-fix detection elements. Active capture-agent on batch-coated bio-elements defeated by shear-force removal or laser-scanning deactivation, so, e.g. inside surface only of hollow elements carries capture-agent. Sample may flow through hollow elements and by-pass outside without assay disturbance.