The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 15, 2015
Filed:
Apr. 28, 2011
Boye Schnack Nielsen, Copenhagen, DK;
Stine Jørgensen, Copenhagen, DK;
Jan Skouv, Espergaerde, DK;
Adam Baker, Vedbaek, DK;
Søren Møller, Holte, DK;
Boye Schnack Nielsen, Copenhagen, DK;
Stine Jørgensen, Copenhagen, DK;
Jan Skouv, Espergaerde, DK;
Adam Baker, Vedbaek, DK;
Søren Møller, Holte, DK;
EXIQON A/S, Vedbaek, DK;
Abstract
An improved method of in situ hybridization which relies on an improved formulation of the in situ hybridization buffer is described. In at least some formulations the buffer are non-toxic. The combination of Locked Nucleic Acid (LNA) comprising ISH probes and the improved ISH buffer are useful for detection of small non-coding RNA as well as in the manufacturing of ISH kits directed to the detection of such small non-coding RNA. Further disclosed is a method of semi-quantitative ISH and demonstration of the semi-quantitative ISHs diagnostic potential.