The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 08, 2015
Filed:
Nov. 14, 2007
Adrien P. Malick, Granite, MD (US);
Virginia M. Crews, Baltimore, MD (US);
Julie L. Rosales, Randallstown, MD (US);
Carrie S. Ferguson, Bel Air, MD (US);
Jeff H. Bruton, Randallstown, MD (US);
Robert J. Beadenkopf, Pasadena, MD (US);
John Mantlo, Westminster, MD (US);
Adrien P. Malick, Granite, MD (US);
Virginia M. Crews, Baltimore, MD (US);
Julie L. Rosales, Randallstown, MD (US);
Carrie S. Ferguson, Bel Air, MD (US);
Jeff H. Bruton, Randallstown, MD (US);
Robert J. Beadenkopf, Pasadena, MD (US);
John Mantlo, Westminster, MD (US);
ARBOR VITA CORPORATION, Fremont, CA (US);
Abstract
Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.