The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 08, 2015

Filed:

Jan. 12, 2010
Applicant:

Masahiko Hase, Tokyo, JP;

Inventor:

Masahiko Hase, Tokyo, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 13/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0062 (2013.01); C12N 2525/00 (2013.01); C12N 2535/10 (2013.01);
Abstract

This invention provides a method for preparing biological tissue in which the thickness (i.e., the number of cell layers) can be easily regulated, culture can be conducted within a shorter period of time than is possible with conventional techniques, and addition of unfavorable components is not necessary. The method of the invention comprises adding a cell-containing culture solution to a culture vessel having a non-cell-adhesive inner bottom surface, conducting cell culture while centrifugal force toward the inner bottom surface is applied to the cells in the vessel, forming tissue via intercellular adhesion, and detaching and collecting the resulting tissue from the inner bottom surface.


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