The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 01, 2015
Filed:
Jul. 20, 2011
Satoshi Yanagi, Shiga, JP;
Eiji Kobayashi, Shiga, JP;
Takashi Uemori, Shiga, JP;
Hiroyuki Mukai, Shiga, JP;
Satoshi Yanagi, Shiga, JP;
Eiji Kobayashi, Shiga, JP;
Takashi Uemori, Shiga, JP;
Hiroyuki Mukai, Shiga, JP;
TAKARA BIO INC., Otsu-Shi, Shiga, JP;
Abstract
A method for accurately and easily detecting a synthetic siRNA, for example, a siRNA in which the 3' end is DNA, and a kit used for the method are provided. The present invention relates to a method for detecting a siRNA in which the 3′ end is DNA, comprising: (a) adding polydeoxyadenosine to the 3′ DNA end of at least one strand of the siRNA to be detected to produce a polydeoxyadenosine-added RNA; (b) annealing a polydeoxythymidine primer having a tag sequence at its 5′ side to the polydeoxyadenosine-added RNA and synthesizing DNA from the primer by a reverse transcription; and (c) detecting the DNA synthesized in (b).