The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 24, 2015

Filed:

Jul. 12, 2012
Applicants:

Sangeeta N. Bhatia, Lexington, MA (US);

Bevin P. Engelward, Lexington, MA (US);

David K. Wood, Cambridge, MA (US);

David M. Weingeist, Cambridge, MA (US);

Inventors:

Sangeeta N. Bhatia, Lexington, MA (US);

Bevin P. Engelward, Lexington, MA (US);

David K. Wood, Cambridge, MA (US);

David M. Weingeist, Cambridge, MA (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 27/447 (2006.01); B01L 3/00 (2006.01);
U.S. Cl.
CPC ...
G01N 27/44782 (2013.01); B01L 3/502715 (2013.01); B01J 2219/0043 (2013.01); B01J 2219/00317 (2013.01); B01J 2219/00527 (2013.01); B01J 2219/00605 (2013.01); B01J 2219/00612 (2013.01); B01J 2219/00637 (2013.01); B01J 2219/00662 (2013.01); B01J 2219/00743 (2013.01); B01L 2300/069 (2013.01); B01L 2300/0809 (2013.01); B01L 2300/0819 (2013.01); B01L 2400/049 (2013.01); B01L 2400/0421 (2013.01);
Abstract

Systems, methods, and devices are provided for assessing DNA damage and repair in cells by measuring DNA migration under electrophoresis. In one exemplary embodiment, a microarray configured to hold cells in a predetermined spatial relationship is employed to improve accuracy, speed, and reliability of such measurements. In another embodiment, a self-contained cassette having a matrix material disposed therein can be used to create a substantially uniform environment for analyzing DNA damage and repair. Fluid can be circulated through the cell to assist in creating spatial patterns on the matrix material, or alternatively, the matrix material can already include a microarray pattern disposed thereon. Various methods and systems that take advantage of such microarrays and cassettes are also provided.


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