The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 24, 2015
Filed:
Mar. 07, 2013
Julianne M Gibbs-davis, Edmonton, CA;
Rosalie Dawn Mckay, Sherwoodpark, CA;
Yimeng LI, Beijing, CN;
Jade Lam, Calgary, CA;
Abu Kausar, Dhaka, BD;
Catherine Jennifer Mitran, Edmonton, CA;
Julianne M Gibbs-Davis, Edmonton, CA;
Rosalie Dawn McKay, Sherwoodpark, CA;
Yimeng Li, Beijing, CN;
Jade Lam, Calgary, CA;
Abu Kausar, Dhaka, BD;
Catherine Jennifer Mitran, Edmonton, CA;
Other;
Abstract
The invention is directed to a method for isothermally amplifying a DNA sequence involving hybridizing a destabilizing DNA template to complementary nucleotide fragments to form a first nicked duplex; ligating the first nicked duplex to form a product duplex comprising the DNA sequence and the template, wherein the product duplex is capable of dissociating to release the DNA sequence and the template; and repeating these steps to generate multiple copies of the template and the DNA sequence. Further, the method may also involve hybridizing the DNA sequence to complementary destabilizing fragments or probes to form a second nicked duplex; ligating the second nicked duplex to form the product duplex comprising the DNA sequence and the template, wherein the product duplex dissociates to release the DNA sequence and the template; and repeating these steps to generate multiple copies of the template and the DNA sequence.