The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 10, 2015

Filed:

Sep. 17, 2014
Applicant:

The Curators of the University of Missouri, Columbia, MO (US);

Inventors:

Venumadhav Korampally, Columbia, MO (US);

Shubhra Gangopadhyay, Columbia, MO (US);

Keshab Gangopadhyay, Columbia, MO (US);

Sheila A. Grant, Columbia, MO (US);

Steven B. Kleiboeker, Lee's Summit, MO (US);

Shantanu Bhattacharya, West Lafayette, IN (US);

Yuanfang Gao, Columbia, MO (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/68 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); B01L 3/5027 (2013.01); B01L 3/502707 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6844 (2013.01); B01L 7/52 (2013.01); B01L 2200/12 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/123 (2013.01); B01L 2300/1827 (2013.01); B01L 2400/0421 (2013.01); B01L 2400/0481 (2013.01);
Abstract

A DNA amplification device utilizing a polydimethylsiloxane (PDMS) and silicon substrate coated with spin-on glass (SOG) is provided. This PDMS layer is irreversibly bonded to the SOG layer of the silicon substrate using oxygen plasma. The amplification device is an inexpensive, microfluidic device, which can be utilized as a portable thermo-cycler to perform PCR amplification of DNA in the field.


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