The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 10, 2015

Filed:

Jan. 10, 2012
Applicants:

Suzanne U. Emerson, Gaithersburg, MD (US);

Priyanka Shukla, Mumbai, IN;

Hanh T. Nguyen, Lanham, MD (US);

Robert H. Purcell, Gaithersburg, MD (US);

Harry R. Dalton, Bodmin, GB;

Richard Bendall, Cornwall, GB;

Inventors:

Suzanne U. Emerson, Gaithersburg, MD (US);

Priyanka Shukla, Mumbai, IN;

Hanh T. Nguyen, Lanham, MD (US);

Robert H. Purcell, Gaithersburg, MD (US);

Harry R. Dalton, Bodmin, GB;

Richard Bendall, Cornwall, GB;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 39/29 (2006.01); A61K 39/12 (2006.01); C07H 21/00 (2006.01); C12N 7/00 (2006.01); C12N 15/86 (2006.01); C12Q 1/70 (2006.01); C07K 14/005 (2006.01); A61K 39/00 (2006.01);
U.S. Cl.
CPC ...
C12N 7/00 (2013.01); C07K 14/005 (2013.01); C12N 15/86 (2013.01); C12Q 1/707 (2013.01); A61K 39/00 (2013.01); C12N 2770/28121 (2013.01); C12N 2770/28122 (2013.01); C12N 2770/28143 (2013.01); C12N 2770/28151 (2013.01);
Abstract

The invention relates to the discovery of an HEV strain from a chronically infected patient. The virus grow unusually well in numerous cell cultures. Thus, the invention provides cell cultures, vectors, and vaccine compositions based on the virus. The invention relates, in part, on the identification of a new strain of HEV genotype 3 virus. Strain Kernow-C1 (genotype 3) of HEV, which was isolated from a chronically infected patient, was used to identify human, pig and deer cell lines permissive for infection. Adaptation of the Kernow-C 1 strain to growth in human hepatoma cells selected for a rare virus recombinant that contained an insertion of 174 ribonucleotides (58 amino acids) of a human ribosomal protein gene and additional mutations.


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