The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 10, 2015
Filed:
Jul. 27, 2012
Alan T. Y. Cheng, Naperville, IL (US);
Ying Zhou, Naperville, IL (US);
Amitabh Gupta, Naperville, IL (US);
Balazs Hunek, Western Springs, IL (US);
Nigel Grinter, Buffalo Grove, IL (US);
Alan T. Y. Cheng, Naperville, IL (US);
Ying Zhou, Naperville, IL (US);
Amitabh Gupta, Naperville, IL (US);
Balazs Hunek, Western Springs, IL (US);
Nigel Grinter, Buffalo Grove, IL (US);
PRAXAIR TECHNOLOGY, INC., Danbury, CT (US);
Abstract
A bioreactor with an upward flowing impeller system for controlling a mammalian cell culture process is provided. The disclosed system enables control of the cell culture process by controlling the level of dissolved carbon dioxide in the cell culture media and prevent increases in the osmolality level is achieved by enhanced stripping of carbon dioxide via surface gas exchange with little or no damage to the mammalian cells. The use of an upward flow impeller combined with vertical baffles converts the swirling motions of the cell culture media into a largely vertical flow and promotes the removal of dissolved carbon dioxide via surface gas exchange with a sweep gas flowing over the top surface of the cell culture media within the bioreactor vessel.