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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 9175296 B1

PDFFull Text
Date of Patent:
Nov. 03, 2015

Filed:

Mar. 16, 2010

Fungal production system

Applicants:

Peter J. Punt, Houten, NL;

Richard Paul Burlingame, Nicholasville, KY (US);

Christine M. Pynnonen, Appleton, WI (US);

Phillip T. Olson, Two Rivers, WI (US);

Jan Wery, Gorssel, NL;

Johannes Heinrich Visser, Wijchen, NL;

Mark A. Emalfarb, Jupiter, FL (US);

Jacob Visser, Wageningen, NL;

Jan Cornelis Verdoes, late of, Wageningen, NL;

Jacoba Verdoes, Katwijk, NL;

Inventors:

Peter J. Punt, Houten, NL;

Richard Paul Burlingame, Nicholasville, KY (US);

Christine M. Pynnonen, Appleton, WI (US);

Phillip T. Olson, Two Rivers, WI (US);

Jan Wery, Gorssel, NL;

Johannes Heinrich Visser, Wijchen, NL;

Mark A. Emalfarb, Jupiter, FL (US);

Jacob Visser, Wageningen, NL;

Jan Cornelis Verdoes, late of, Wageningen, NL;

Assignee:

DYADIC NEDERLAND B.V., Wageningen, NL;

Attorney:

Browdy and Neimark, PLLC

Primary Examiner:

Nashaat Nashed

Int. Cl.
CPC ...
C12P 21/00 (2006.01); C12N 15/80 (2006.01); C12N 1/14 (2006.01); C12N 9/12 (2006.01); C12N 9/34 (2006.01); C12N 15/01 (2006.01); C12P 21/02 (2006.01); C12R 1/645 (2006.01); C12N 9/42 (2006.01); C12N 9/24 (2006.01);
U.S. Cl.
CPC ...
C12N 15/80 (2013.01); C12N 1/14 (2013.01); C12N 9/1205 (2013.01); C12N 9/2402 (2013.01); C12N 9/248 (2013.01); C12N 9/2428 (2013.01); C12N 9/2437 (2013.01); C12N 9/2442 (2013.01); C12N 15/01 (2013.01); C12P 21/02 (2013.01); C12R 1/645 (2013.01); C12Y 302/01003 (2013.01); C12Y 302/01004 (2013.01); C12Y 302/01006 (2013.01); C12Y 302/01008 (2013.01); C12Y 302/01014 (2013.01); C12Y 302/01015 (2013.01); C12Y 302/01091 (2013.01); C12Y 304/21062 (2013.01); C12Y 304/23025 (2013.01);
Abstract

The present invention provides a new fungal production system comprising a fungal host strain ofwherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion ofstrain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous β-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion ofstrain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed. Finally a method for simplified screening of strains functionally expressing a desired enzyme by application of said strains have been provided.

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