The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 27, 2015

Filed:

May. 09, 2011
Applicants:

Yuko Nakabayashi, Otsu, JP;

Takashi Uemori, Otsu, JP;

Hiroyuki Mukai, Otsu, JP;

Kiyozo Asada, Otsu, JP;

Inventors:

Yuko Nakabayashi, Otsu, JP;

Takashi Uemori, Otsu, JP;

Hiroyuki Mukai, Otsu, JP;

Kiyozo Asada, Otsu, JP;

Assignee:

TAKARA BIO INC., Otsu-Shi, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1096 (2013.01); C12P 19/34 (2013.01);
Abstract

A method for synthesizing cDNA characterized by preparing a reaction solution that does not allow an endodeoxyribonuclease to show its activity, without thermal deactivation of the endodeoxyribonuclease or removal of the endodeoxyribonuclease, and carrying out a reverse transcription reaction, wherein the reaction solution contains a treated sample and a reverse transcriptase, the treated sample being formed by treating a sample comprising RNA and DNA with the endodeoxyribonuclease to degrade DNA in the sample. The method and the kit for synthesizing cDNA of the present invention are widely useful in genetic engineering fields.


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