The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 20, 2015
Filed:
May. 17, 2013
Liquid tissue preparation from histopathologically processed biologically samples, tissues and cells
Applicant:
Expression Pathology, Inc., Rockville, MD (US);
Inventors:
Marlene M. Darfler, Gaithersburg, MD (US);
David B. Krizman, Gaithersburg, MD (US);
Assignee:
EXPRESSION PATHOLOGY, INC., Rockville, MD (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12M 3/00 (2006.01); G01N 33/53 (2006.01); C12Q 1/37 (2006.01); C12N 15/10 (2006.01); G01N 1/44 (2006.01); C40B 30/04 (2006.01); C40B 40/06 (2006.01); C40B 40/08 (2006.01); G01N 1/30 (2006.01); G01N 1/40 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/37 (2013.01); C12N 15/1003 (2013.01); G01N 1/44 (2013.01); G01N 1/30 (2013.01); G01N 1/4044 (2013.01);
Abstract
The current invention provides a method for directly converting histopathologically processed biological samples, tissues, and cells into a multi-use biomolecule lysate. This method allows for simultaneous extraction, isolation, solublization, and storage of all biomolecules contained within the histopathologically processed biological sample, thereby forming a representative library of said sample. This multi-use biomolecule lysate is dilutable, soluble, capable of being fractionated, and used in any number of subsequent experiments.